{"id":6642,"date":"2020-08-18T09:51:52","date_gmt":"2020-08-18T07:51:52","guid":{"rendered":"https:\/\/outreach-wp.ixole.es\/?post_type=project&p=6642\/"},"modified":"2020-08-23T14:39:39","modified_gmt":"2020-08-23T12:39:39","slug":"autoimmune-encephalitis-and-super-resolution-fluorescence-%e2%80%90-microscopy","status":"publish","type":"project","link":"https:\/\/outreach-wp.ixole.es\/es\/project\/autoimmune-encephalitis-and-super-resolution-fluorescence-%e2%80%90-microscopy\/","title":{"rendered":"Autoimmune Encephalitis and Super Resolution Fluorescence \u2010 Microscopy"},"content":{"rendered":"

[et_pb_section fb_built=\u00bb1″ admin_label=\u00bbsection\u00bb _builder_version=\u00bb4.5.1″ background_color=\u00bb#e1e1e1″ custom_padding=\u00bb0px||0px|\u00bb transparent_background=\u00bboff\u00bb make_fullwidth=\u00bboff\u00bb use_custom_width=\u00bboff\u00bb width_unit=\u00bbon\u00bb][et_pb_row column_structure=\u00bb1_3,1_3,1_3″ use_custom_gutter=\u00bbon\u00bb gutter_width=\u00bb1″ make_equal=\u00bbon\u00bb admin_label=\u00bbrow\u00bb module_class=\u00bb et_pb_row_fullwidth\u00bb _builder_version=\u00bb4.5.1″ background_size=\u00bbinitial\u00bb background_position=\u00bbtop_left\u00bb background_repeat=\u00bbrepeat\u00bb width=\u00bb100%\u00bb width_tablet=\u00bb100%\u00bb width_phone=\u00bb\u00bb width_last_edited=\u00bbon|desktop\u00bb max_width=\u00bb100%\u00bb max_width_tablet=\u00bb100%\u00bb max_width_phone=\u00bb\u00bb max_width_last_edited=\u00bbon|desktop\u00bb custom_padding=\u00bb0px||0px|\u00bb make_fullwidth=\u00bbon\u00bb use_custom_width=\u00bboff\u00bb width_unit=\u00bbon\u00bb][et_pb_column type=\u00bb1_3″ _builder_version=\u00bb4.5.1″ background_color=\u00bb#3b4249″ custom_padding=\u00bb45px|45px|22.5px|45px|false|false\u00bb custom_padding__hover=\u00bb|||\u00bb][et_pb_post_title meta=\u00bboff\u00bb featured_image=\u00bboff\u00bb _builder_version=\u00bb4.5.6″ _module_preset=\u00bbdefault\u00bb title_text_color=\u00bb#d7ac2f\u00bb][\/et_pb_post_title][\/et_pb_column][et_pb_column type=\u00bb1_3″ _builder_version=\u00bb4.5.1″ background_color=\u00bb#d1d1cb\u00bb custom_padding=\u00bb45px|45px|22.5px|45px|false|false\u00bb custom_padding__hover=\u00bb|||\u00bb][et_pb_text admin_label=\u00bbTexto\u00bb _builder_version=\u00bb3.27.4″ background_size=\u00bbinitial\u00bb background_position=\u00bbtop_left\u00bb background_repeat=\u00bbrepeat\u00bb use_border_color=\u00bboff\u00bb border_color=\u00bb#ffffff\u00bb border_style=\u00bbsolid\u00bb]<\/p>\n

Adri\u00e1n barreros p\u00e9rez<\/span><\/h3>\n

<\/h3>\n

[\/et_pb_text][\/et_pb_column][et_pb_column type=\u00bb1_3″ _builder_version=\u00bb4.5.1″ background_color=\u00bb#d1d1cb\u00bb custom_padding=\u00bb45px|45px|22.5px|45px|false|false\u00bb custom_padding__hover=\u00bb|||\u00bb][et_pb_text admin_label=\u00bbTexto\u00bb _builder_version=\u00bb4.5.1″ background_size=\u00bbinitial\u00bb background_position=\u00bbtop_left\u00bb background_repeat=\u00bbrepeat\u00bb use_border_color=\u00bboff\u00bb border_color=\u00bb#ffffff\u00bb border_style=\u00bbsolid\u00bb]<\/p>\n

The aim of this research as a school project was being able to know and experience more about biomedical applications of fluorescence used in scientific research. After applying for some help to ICFO this project was then focused on following the Autoimmune Encephalitis research of ICFO group Advanced Fluorescence Imaging and Biophysics in collaboration with Josep Dalmau at IDIBAPS.
Autoimmune Encephalitis (AE) is an inflammation of the brain caused through the immune system. It produces neuropsychiatric symptoms and has been diagnosed most frequently in young women. It can be
treated but a bad diagnosis can lead to death. There are different types of AE attending to the synapse receptor or protein attacked. I was able to follow the investigations of two of these types:
\u2022 Autoimmune Encephalitis AntiNMDA: the immune system attacks NMDA receptors, causing psychiatric symptoms, confusion and memory loss.
\u2022 Limbic Encephalitis: the immune system attacks the synaptic protein LGI1 which makes a synaptic port between ADAM22 ADM 23 receptors.
STORM is a superresolution fluorescence microscopy method that uses photoswitchable fluorophores to localize single molecules in a sample with a high density of labeling. The imaging process consists of many
cycles during which optically resolvable fluorophores from the rest are activated, imaged and deactivated.
This allows the position of every fluorophore to be determined with nanometer precision to construct a superresolution image.
STORM imaging is being used to discover how and why patients develop the production of antibodies causing
the encephalitis. This is made by comparing normal neurons and treated neurons with patient’s cerebral
spinal fluid. These neurons are obtained from rats and follow this process: primary culture, staining, STORM
imaging.<\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=\u00bb4.5.1″ _module_preset=\u00bbdefault\u00bb background_color=\u00bb#e1e1e1″][et_pb_column type=\u00bb4_4″ _builder_version=\u00bb4.5.1″ _module_preset=\u00bbdefault\u00bb background_color=\u00bb#e1e1e1″][et_pb_image src=\u00bbhttps:\/\/outreach-wp.ixole.es\/wp-content\/uploads\/2020\/08\/Barreros.png\u00bb title_text=\u00bbBarreros\u00bb url=\u00bbhttps:\/\/outreach-wp.ixole.es\/wp-content\/uploads\/2020\/08\/Barreros.png\u00bb align=\u00bbcenter\u00bb force_fullwidth=\u00bbon\u00bb _builder_version=\u00bb4.5.7″ _module_preset=\u00bbdefault\u00bb hover_enabled=\u00bb0″][\/et_pb_image][et_pb_post_nav _builder_version=\u00bb4.5.1″ _module_preset=\u00bbdefault\u00bb title_font=\u00bb|600|||||||\u00bb title_text_color=\u00bb#918050″][\/et_pb_post_nav][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=\u00bb1_3,1_3,1_3″ use_custom_gutter=\u00bbon\u00bb gutter_width=\u00bb1″ make_equal=\u00bbon\u00bb admin_label=\u00bbrow\u00bb module_class=\u00bb et_pb_row_fullwidth\u00bb _builder_version=\u00bb4.5.1″ background_size=\u00bbinitial\u00bb background_position=\u00bbtop_left\u00bb background_repeat=\u00bbrepeat\u00bb width=\u00bb100%\u00bb width_tablet=\u00bb100%\u00bb width_phone=\u00bb\u00bb width_last_edited=\u00bbon|desktop\u00bb max_width=\u00bb100%\u00bb max_width_tablet=\u00bb100%\u00bb max_width_phone=\u00bb\u00bb max_width_last_edited=\u00bbon|desktop\u00bb custom_padding=\u00bb0px||0px|\u00bb make_fullwidth=\u00bbon\u00bb use_custom_width=\u00bboff\u00bb width_unit=\u00bbon\u00bb][et_pb_column type=\u00bb1_3″ _builder_version=\u00bb4.5.1″ background_color=\u00bb#e1e1e1″ custom_padding=\u00bb|45px||45px|false|false\u00bb custom_padding__hover=\u00bb|||\u00bb][\/et_pb_column][et_pb_column type=\u00bb1_3″ _builder_version=\u00bb4.5.1″ background_color=\u00bb#e1e1e1″ custom_padding=\u00bb|45px||45px|false|false\u00bb custom_padding__hover=\u00bb|||\u00bb][\/et_pb_column][et_pb_column type=\u00bb1_3″ _builder_version=\u00bb4.5.1″ background_color=\u00bb#e1e1e1″ custom_padding=\u00bb|45px||45px|false|false\u00bb custom_padding__hover=\u00bb|||\u00bb][et_pb_button button_url=\u00bb@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoicG9zdF9saW5rX3VybF9wb3N0Iiwic2V0dGluZ3MiOnsicG9zdF9pZCI6IjYzNjUifX0=@\u00bb button_text=\u00bbBack to the projects\u00bb button_alignment=\u00bbleft\u00bb admin_label=\u00bbBot\u00f3n Dorado\u00bb module_id=\u00bbbotons\u00bb module_class=\u00bbbotons\u00bb _builder_version=\u00bb4.5.7″ _dynamic_attributes=\u00bbbutton_url\u00bb custom_button=\u00bbon\u00bb button_text_color=\u00bb#918050″ button_border_color=\u00bb#918050″ button_icon_color=\u00bb#918050″ button_on_hover=\u00bboff\u00bb custom_css_main_element=\u00bbmargin-top: 10px;\u00bb custom_css_after=\u00bb margin-left: 0px !important;\u00bb button_text_color_hover=\u00bb#918050″ button_border_color_hover=\u00bb#918050″ button_border_radius_hover=\u00bb0″ button_letter_spacing_hover=\u00bb0″ saved_tabs=\u00bball\u00bb button_text_size__hover_enabled=\u00bboff\u00bb button_one_text_size__hover_enabled=\u00bboff\u00bb button_two_text_size__hover_enabled=\u00bboff\u00bb button_text_color__hover_enabled=\u00bbon\u00bb button_text_color__hover=\u00bb#918050″ button_one_text_color__hover_enabled=\u00bboff\u00bb button_two_text_color__hover_enabled=\u00bboff\u00bb button_border_width__hover_enabled=\u00bboff\u00bb button_one_border_width__hover_enabled=\u00bboff\u00bb button_two_border_width__hover_enabled=\u00bboff\u00bb button_border_color__hover_enabled=\u00bbon\u00bb button_border_color__hover=\u00bb#918050″ button_one_border_color__hover_enabled=\u00bboff\u00bb button_two_border_color__hover_enabled=\u00bboff\u00bb button_border_radius__hover_enabled=\u00bbon\u00bb button_border_radius__hover=\u00bb0″ button_one_border_radius__hover_enabled=\u00bboff\u00bb button_two_border_radius__hover_enabled=\u00bboff\u00bb button_letter_spacing__hover_enabled=\u00bbon\u00bb button_letter_spacing__hover=\u00bb0″ button_one_letter_spacing__hover_enabled=\u00bboff\u00bb button_two_letter_spacing__hover_enabled=\u00bboff\u00bb button_bg_color__hover_enabled=\u00bboff\u00bb button_one_bg_color__hover_enabled=\u00bboff\u00bb button_two_bg_color__hover_enabled=\u00bboff\u00bb][\/et_pb_button][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"

Adri\u00e1n barreros p\u00e9rez The aim of this research as a school project was being able to know and experience more about biomedical applications of fluorescence used in scientific research. After applying for some help to ICFO this project was then focused on following the Autoimmune Encephalitis research of ICFO group Advanced Fluorescence Imaging and Biophysics […]<\/p>\n","protected":false},"author":4,"featured_media":0,"comment_status":"open","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":""},"project_category":[225,226,227,228],"project_tag":[],"acf":[],"yoast_head":"\nAutoimmune Encephalitis and Super Resolution Fluorescence \u2010 Microscopy - Outreach<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/outreach-wp.ixole.es\/es\/project\/autoimmune-encephalitis-and-super-resolution-fluorescence-\u2010-microscopy\/\" \/>\n<meta property=\"og:locale\" content=\"es_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Autoimmune Encephalitis and Super Resolution Fluorescence \u2010 Microscopy - Outreach\" \/>\n<meta property=\"og:description\" content=\"Adri\u00e1n barreros p\u00e9rez The aim of this research as a school project was being able to know and experience more about biomedical applications of fluorescence used in scientific research. After applying for some help to ICFO this project was then focused on following the Autoimmune Encephalitis research of ICFO group Advanced Fluorescence Imaging and Biophysics […]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/outreach-wp.ixole.es\/es\/project\/autoimmune-encephalitis-and-super-resolution-fluorescence-\u2010-microscopy\/\" \/>\n<meta property=\"og:site_name\" content=\"Outreach\" \/>\n<meta property=\"article:modified_time\" content=\"2020-08-23T12:39:39+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Tiempo de lectura\" \/>\n\t<meta name=\"twitter:data1\" content=\"5 minutos\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/outreach-wp.ixole.es\/es\/project\/autoimmune-encephalitis-and-super-resolution-fluorescence-%e2%80%90-microscopy\/\",\"url\":\"https:\/\/outreach-wp.ixole.es\/es\/project\/autoimmune-encephalitis-and-super-resolution-fluorescence-%e2%80%90-microscopy\/\",\"name\":\"Autoimmune Encephalitis and Super Resolution Fluorescence \u2010 Microscopy - Outreach\",\"isPartOf\":{\"@id\":\"https:\/\/outreach-wp.ixole.es\/#website\"},\"datePublished\":\"2020-08-18T07:51:52+00:00\",\"dateModified\":\"2020-08-23T12:39:39+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/outreach-wp.ixole.es\/es\/project\/autoimmune-encephalitis-and-super-resolution-fluorescence-%e2%80%90-microscopy\/#breadcrumb\"},\"inLanguage\":\"es\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/outreach-wp.ixole.es\/es\/project\/autoimmune-encephalitis-and-super-resolution-fluorescence-%e2%80%90-microscopy\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/outreach-wp.ixole.es\/es\/project\/autoimmune-encephalitis-and-super-resolution-fluorescence-%e2%80%90-microscopy\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Portada\",\"item\":\"https:\/\/outreach-wp.ixole.es\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Projects\",\"item\":\"https:\/\/outreach-wp.ixole.es\/ca\/project\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Autoimmune Encephalitis and Super Resolution Fluorescence \u2010 Microscopy\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/outreach-wp.ixole.es\/#website\",\"url\":\"https:\/\/outreach-wp.ixole.es\/\",\"name\":\"Outreach\",\"description\":\"ICFO's Outreach\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/outreach-wp.ixole.es\/?s={search_term_string}\"},\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"es\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Autoimmune Encephalitis and Super Resolution Fluorescence \u2010 Microscopy - Outreach","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/outreach-wp.ixole.es\/es\/project\/autoimmune-encephalitis-and-super-resolution-fluorescence-\u2010-microscopy\/","og_locale":"es_ES","og_type":"article","og_title":"Autoimmune Encephalitis and Super Resolution Fluorescence \u2010 Microscopy - Outreach","og_description":"Adri\u00e1n barreros p\u00e9rez The aim of this research as a school project was being able to know and experience more about biomedical applications of fluorescence used in scientific research. After applying for some help to ICFO this project was then focused on following the Autoimmune Encephalitis research of ICFO group Advanced Fluorescence Imaging and Biophysics […]","og_url":"https:\/\/outreach-wp.ixole.es\/es\/project\/autoimmune-encephalitis-and-super-resolution-fluorescence-\u2010-microscopy\/","og_site_name":"Outreach","article_modified_time":"2020-08-23T12:39:39+00:00","twitter_card":"summary_large_image","twitter_misc":{"Tiempo de lectura":"5 minutos"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/outreach-wp.ixole.es\/es\/project\/autoimmune-encephalitis-and-super-resolution-fluorescence-%e2%80%90-microscopy\/","url":"https:\/\/outreach-wp.ixole.es\/es\/project\/autoimmune-encephalitis-and-super-resolution-fluorescence-%e2%80%90-microscopy\/","name":"Autoimmune Encephalitis and Super Resolution Fluorescence \u2010 Microscopy - Outreach","isPartOf":{"@id":"https:\/\/outreach-wp.ixole.es\/#website"},"datePublished":"2020-08-18T07:51:52+00:00","dateModified":"2020-08-23T12:39:39+00:00","breadcrumb":{"@id":"https:\/\/outreach-wp.ixole.es\/es\/project\/autoimmune-encephalitis-and-super-resolution-fluorescence-%e2%80%90-microscopy\/#breadcrumb"},"inLanguage":"es","potentialAction":[{"@type":"ReadAction","target":["https:\/\/outreach-wp.ixole.es\/es\/project\/autoimmune-encephalitis-and-super-resolution-fluorescence-%e2%80%90-microscopy\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/outreach-wp.ixole.es\/es\/project\/autoimmune-encephalitis-and-super-resolution-fluorescence-%e2%80%90-microscopy\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Portada","item":"https:\/\/outreach-wp.ixole.es\/"},{"@type":"ListItem","position":2,"name":"Projects","item":"https:\/\/outreach-wp.ixole.es\/ca\/project\/"},{"@type":"ListItem","position":3,"name":"Autoimmune Encephalitis and Super Resolution Fluorescence \u2010 Microscopy"}]},{"@type":"WebSite","@id":"https:\/\/outreach-wp.ixole.es\/#website","url":"https:\/\/outreach-wp.ixole.es\/","name":"Outreach","description":"ICFO's Outreach","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/outreach-wp.ixole.es\/?s={search_term_string}"},"query-input":"required name=search_term_string"}],"inLanguage":"es"}]}},"_links":{"self":[{"href":"https:\/\/outreach-wp.ixole.es\/es\/wp-json\/wp\/v2\/project\/6642"}],"collection":[{"href":"https:\/\/outreach-wp.ixole.es\/es\/wp-json\/wp\/v2\/project"}],"about":[{"href":"https:\/\/outreach-wp.ixole.es\/es\/wp-json\/wp\/v2\/types\/project"}],"author":[{"embeddable":true,"href":"https:\/\/outreach-wp.ixole.es\/es\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/outreach-wp.ixole.es\/es\/wp-json\/wp\/v2\/comments?post=6642"}],"version-history":[{"count":5,"href":"https:\/\/outreach-wp.ixole.es\/es\/wp-json\/wp\/v2\/project\/6642\/revisions"}],"predecessor-version":[{"id":7104,"href":"https:\/\/outreach-wp.ixole.es\/es\/wp-json\/wp\/v2\/project\/6642\/revisions\/7104"}],"wp:attachment":[{"href":"https:\/\/outreach-wp.ixole.es\/es\/wp-json\/wp\/v2\/media?parent=6642"}],"wp:term":[{"taxonomy":"project_category","embeddable":true,"href":"https:\/\/outreach-wp.ixole.es\/es\/wp-json\/wp\/v2\/project_category?post=6642"},{"taxonomy":"project_tag","embeddable":true,"href":"https:\/\/outreach-wp.ixole.es\/es\/wp-json\/wp\/v2\/project_tag?post=6642"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}